Safety and efficacy of combined administration of Porcilis PRRS and other vaccines in pigs

Combined administration of Porcilis PRRS and Porcilis Ery+Parvo

The efficacy of  simultaneous use of Porcilis PRRS and Porcilis Parvo+Ery was measured by the serological response of the pigs. The subsequent reproductive performance of the gilts was monitored.

Method

  • Group 1: 19 gilts, were vaccinated with Porcilis PRRS and Porcilis Ery + Parvo simultaneously with one syringe, in which both vaccines had been mixed immediately prior to use.
  • Group 2: 21 gilts, were vaccinated with Porcilis PRRS and Porcilis Ery + Parvo at two different sites, using two separate syringes.
  • Animals were monitored for local and systemic side effects for 1-2 hours post injection and at 24 hours.
  • Blood samples were taken at two time points: at Day 0 (T0), and Day 28 (T1) and assayed to assess seroconversion for PRRS by immunofluorescence testing (IFT) and for Parvovirus by Haeminhibition test (HI).
  • Both groups were followed until farrowing and reproductive parameters (abortions, live born piglets etc.) were compared

Results
 No local or systemic reactions were seen in either group post vaccination.

combined vaccination

Graph 1: Parvovirus HI: average values. The initial HI titre (T0) to porcine parvovirus was high in both groups, probably due to a previous field infection. At T1 an increase in the average Parvovirus HI was registered in both Groups.

combined vaccination

Graph 2: PRRS IFT: average values. At T0 the titres were below detection level.

There was no difference in reproductive performance between Groups 1 and 2 (data not shown).

Conclusion
Simultaneous application of Porcilis PRRS with Porcilis Ery + Parvo has no effect on PRRS seroconversion or subsequent reproductive performance.

Combined administration of Porcilis PRRS and Porcilis Begonia

Current vaccination instructions prescribes that administration Porcilis PRRS Porcilis Begonia (Aujeszky’s disease virus (ADV) vaccine) be separated by an interval of two weeks. However, in the field simultaneous application of two vaccines is common practice.

The purpose of this study is to verify experimentally the clinical efficacy of this practice, as measured by serum titres to each vaccine.

Method
 The study was conducted in a quarantine house with ADV and PRRSV free pigs. The pigs had previously received an ADV vaccination with a gE deleted vaccine.

  • Group 1: 11 gilts were given the recommended doses of Porcilis PRRS and Porcilis Begonia vaccines, mixed within the same syringe (simultaneous use).
  • Group 2: 16 gilts were given two separate injections (concurrent use), one of each vaccine, in different sites in the neck.
  • Blood samples were taken from all pigs on the day of vaccination (T0) and 35 days later (T1) and assayed for titres to ADV and PRRSV (serum neutralising (SN) titres for the ADV and Idexx PRRS ELISA kit for PRRS).
  • All animals were subjected to a daily clinical check from day 0 to day 35, and the following clinical parameters were scored: body temperature, dyspnoea and lethargy.

Results
No local or systemic reactions were seen in either group post vaccination.

combined vaccination

Graph 3: ADV SN: average values

combined vaccination

Graph 4: PRRS ELISA: average values. At T0 the titres were below detection level.

Conclusion
There was no difference in local or systemic reactions or seroconversion between the two groups, suggesting that mixing the two vaccines within the same syringe is a safe and efficacious practice

Porcilis PRRS